After the death of Alexander the Great, Ephesus was ruled by the general of him, Lysimakhos, in 287 BC. Lysimakhos decided to change the prior location of Ephesus to further west, due to the destruction of the port by the alluviums, and the inhabitants were forced to settle in the new place named "Arsinoeina", the name of Lysimakhos’ wife.

  
The city was surrounded by wide stone walls in 10 meters height and 9 meters length. With the death of Lysimakhos, Ephesians destroyed most of the city walls. And, "Arsinoeina" was changed into "Ephesus" again, to be forgotten eternally. Ephesus was controlled by the Romans in 190 BC. The city was given to the Bergamian kings for a time.  
  
With the death of King Attalos 3 in 133BC, the city was re-ruled by the Romans. Ephesus reached to its height and was notorious for its wealth and luxury between 1-4 AD., especially during the reign of Augustus. During the period, the population of Ephesus increased to 225 000, and the city became the capital of the new Asia.

By cleaning the river Caystros from the alluviums, the great trade port of Ephesus, a gateway to foreign countries, enriched the prosperity of the city and continued to thrive with commerce and culture. The city was constructed, adding new models to the former magnificence of Ephesus. "Celcius Library" clearly exemplifies the perfecta of the era, with the delicate details of the construction.

Ephesus has played significant roles during the date, in the early Christianity, as well. The prestige of Ephesus increased with the arrival of Saint Paul, for spreading the Christianity to the Ephesians worshipping to Artemision. St. Paul and the disciplines of Christianity were strictly refused by Ephesians, elderly. With the long tiring struggles of St. Paul, Christianity was accepted by the most of the population around Ephesus.